Algorithms , Autoanalysis/methods , Blood Cell Count/methods , Erythrocytes/metabolism , Reticulocytes/metabolism , Spherocytosis, Hereditary/diagnosis , Adolescent , Adult , Aged , Autoanalysis/instrumentation , Blood Cell Count/instrumentation , Child , Child, Preschool , Diagnostic Tests, Routine/instrumentation , Diagnostic Tests, Routine/methods , Female , Humans , Infant , Infant, Newborn , Male , Middle Aged , Reproducibility of Results , Sensitivity and Specificity , Spherocytosis, Hereditary/blood , Young Adult
Large granular lymphocyte leukemia (LGL) are chronic lymphoproliferative disorders classified into three main groups: T-cell LGL leukemia (T-LGL), aggressive NK-cell leukemia and chronic lymphoproliferative disorder of NK cells (NK-LGL). Patients with LGL leukemia exhibit chronic (>3 months) and moderate (<1G/L) to substantial monoclonal expansion of large granular lymphocytes in the peripheral blood. Cytologically, large granular lymphocytes are medium to large cells which are further characterized by an eccentric nucleus and a slightly basophilic cytoplasm containing azurophilic granules. Typically, T-LGL (CD3-and mostly CD8+) can be differentiated from NK-LGL disorders (CD3-) based on flow cytometry analysis. However, distinction between LGL leukemias can be tricky. We report here the case of a 47-year-old woman patient diagnosed with large granular lymphocytes leukemia associated with atypical CD3-CD56- immunophenotyping and clinical manifestations of pseudo-Felty's syndrome.
CD3 Complex/metabolism , CD56 Antigen/metabolism , Leukemia, Large Granular Lymphocytic/diagnosis , Diagnosis, Differential , Female , Flow Cytometry , Humans , Immunophenotyping , Killer Cells, Natural/metabolism , Killer Cells, Natural/pathology , Leukemia, Large Granular Lymphocytic/blood , Leukemia, Large Granular Lymphocytic/pathology , Medical Laboratory Personnel , Middle Aged , Physicians
